Assay Detail
Binding
CHEMBL667319
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In vitro inhibition of dihydrofolate reductase (DHFR) in L1210 cells
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | L1210 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Synthesis and antifolate evaluation of 10-ethyl-5-methyl-5,10- dideazaaminopterin and an alternative synthesis of 10-ethyl-10- deazaaminopterin (edatrexate).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 2 | 10.00 | 10.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL9798 | — | — | 1 | 10.00 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL9798 | — | Ki | = | 0.1 | nM | 10.00 |
| CHEMBL34259 | METHOTREXATE | Ki | = | 0.00482 | nM | - |