Assay Detail
Binding
CHEMBL667322
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Evaluated for inhibition of dihydrofolate reductase (DHFR) isolated from L1210 cells
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | L1210 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Synthesis and antifolate activity of 5-methyl-5,10-dideaza analogues of aminopterin and folic acid and an alternative synthesis of 5,10-dideazatetrahydrofolic acid, a potent inhibitor of glycinamide ribonucleotide formyltransferase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 5 | 10.55 | 10.55 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL69016 | — | — | 1 | 10.55 |
| CHEMBL66864 | — | — | 1 | - |
| CHEMBL267890 | — | — | 1 | - |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | - |
| CHEMBL66389 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL69016 | — | Ki | = | 0.0283 | nM | 10.55 |
| CHEMBL66864 | — | Ki | > | 1.0 | nM | - |
| CHEMBL267890 | — | Ki | > | 1.0 | nM | - |
| CHEMBL34259 | METHOTREXATE | Ki | = | 0.00545 | nM | - |
| CHEMBL66389 | — | Ki | > | 1.0 | nM | - |