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Assay Detail

CHEMBL667325

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of the dihydrofolate reductase enzyme(DHFR) derived from L1210 murine leukemia cells.
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL4564)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and antifolate properties of 5,10-ethano-5,10-dideazaaminopterin.
DeGraw JI, Christie PH, Colwell WT, Sirotnak FM.
J Med Chem (1992) 35 : 320 -324
PubMed 1732549 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 10.83 10.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL113799 1 10.89
CHEMBL109229 1 10.77
CHEMBL34259 METHOTREXATE 4.0 1 -
CHEMBL110251 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL113799 Ki = 0.013 nM 10.89
CHEMBL109229 Ki = 0.017 nM 10.77
CHEMBL34259 METHOTREXATE Ki = 0.006 nM -
CHEMBL110251 Ki = 0.007 nM -