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Assay Detail

CHEMBL667326

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity of Dihydrofolate reductase derived from L1210 cell line.
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL4564)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and antifolate properties of 10-alkyl-5,10-dideaza analogues of methotrexate and tetrahydrofolic acid.
DeGraw JI, Christie PH, Kisliuk RL, Gaumont Y, Sirotnak FM.
J Med Chem (1990) 33 : 673 -677
PubMed 2299633 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 10.89 10.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL113799 1 10.89
CHEMBL34259 METHOTREXATE 4.0 1 -
CHEMBL162414 1 -
CHEMBL279508 1 -
CHEMBL162701 1 -
CHEMBL110251 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL113799 Ki = 0.013 nM 10.89
CHEMBL34259 METHOTREXATE Ki = 0.006 nM -
CHEMBL162414 Ki > 1000.0 nM -
CHEMBL279508 Ki > 1000.0 nM -
CHEMBL162701 Ki > 1000.0 nM -
CHEMBL110251 Ki = 0.007 nM -