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Assay Detail

CHEMBL667766

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Binding
Inhibitory activity against dihydrofolate reductase (DHFR) of Plasmodium berghei
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium berghei
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL3963)
Type SINGLE PROTEIN
Organism Plasmodium berghei str. ANKA

Publication

2,4-Diamino-5-benzylpyrimidines as antibacterial agents. 7. Analysis of the effect of 3,5-dialkyl substituent size and shape on binding to four different dihydrofolate reductase enzymes.
Roth B, Rauckman BS, Ferone R, Baccanari DP, Champness JN, Hyde RM.
J Med Chem (1987) 30 : 348 -356
PubMed 3100802 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.50 8.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL440334 1 8.24
CHEMBL23746 1 8.24
CHEMBL116183 1 8.12
CHEMBL118877 1 8.00
CHEMBL279455 1 8.00
CHEMBL23250 1 7.92
CHEMBL24374 1 7.89
CHEMBL25888 1 7.82
CHEMBL26337 1 7.70
CHEMBL280374 1 7.64
CHEMBL25817 1 7.55
CHEMBL120366 1 7.51
CHEMBL116993 1 7.47
CHEMBL332481 1 7.46
CHEMBL118367 1 7.38
CHEMBL118480 1 7.28
CHEMBL24155 1 7.11
CHEMBL440946 1 6.96
CHEMBL23447 1 6.92
CHEMBL287553 1 6.92
CHEMBL22 TRIMETHOPRIM 4.0 1 6.92
CHEMBL277176 1 6.85
CHEMBL1181 4-HYDROXYTRIMETHOPRIM 1 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL440334 IC50 = 5.8 nM 8.24
CHEMBL23746 IC50 = 5.7 nM 8.24
CHEMBL116183 IC50 = 7.5 nM 8.12
CHEMBL118877 IC50 = 10.0 nM 8.00
CHEMBL279455 IC50 = 10.0 nM 8.00
CHEMBL23250 IC50 = 12.1 nM 7.92
CHEMBL24374 IC50 = 13.0 nM 7.89
CHEMBL25888 IC50 = 15.0 nM 7.82
CHEMBL26337 IC50 = 20.0 nM 7.70
CHEMBL280374 IC50 = 23.0 nM 7.64
CHEMBL25817 IC50 = 28.0 nM 7.55
CHEMBL120366 IC50 = 31.0 nM 7.51
CHEMBL116993 IC50 = 34.0 nM 7.47
CHEMBL332481 IC50 = 35.0 nM 7.46
CHEMBL118367 IC50 = 42.0 nM 7.38
CHEMBL118480 IC50 = 53.0 nM 7.28
CHEMBL24155 IC50 = 77.0 nM 7.11
CHEMBL440946 IC50 = 110.0 nM 6.96
CHEMBL23447 IC50 = 120.0 nM 6.92
CHEMBL287553 IC50 = 120.0 nM 6.92
CHEMBL22 TRIMETHOPRIM IC50 = 120.0 nM 6.92
CHEMBL277176 IC50 = 140.0 nM 6.85
CHEMBL1181 4-HYDROXYTRIMETHOPRIM IC50 = 300.0 nM 6.52