Assay Detail
Binding
CHEMBL668083
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibitory concentration against recombinant human DHFR(Dihydro folate reductase).
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Effect of bridge region variation on antifolate and antitumor activity of classical 5-substituted 2,4-diaminofuro[2,3-d]pyrimidines.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.58 | 7.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.40 |
| CHEMBL106628 | — | — | 1 | 6.35 |
| CHEMBL323565 | — | — | 1 | 6.00 |
| CHEMBL106699 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL106628 | — | IC50 | = | 450.0 | nM | 6.35 |
| CHEMBL323565 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL106699 | — | IC50 | > | 200000.0 | nM | - |