Assay Detail
Binding
CHEMBL668094
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was tested for its inhibitory activity on Recombinant Human Dihydrofolate Reductase.
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Folate analogues. 34. Synthesis and antitumor activity of non-polyglutamylatable inhibitors of dihydrofolate reductase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 8.27 | 8.41 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 8.41 |
| CHEMBL293263 | — | — | 1 | 8.39 |
| CHEMBL296373 | EDATREXATE | 2.0 | 1 | 8.36 |
| CHEMBL307820 | — | — | 1 | 8.28 |
| CHEMBL308279 | — | — | 1 | 8.24 |
| CHEMBL73267 | — | — | 1 | 8.23 |
| CHEMBL65616 | — | — | 1 | 8.19 |
| CHEMBL307486 | — | — | 1 | 8.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 3.9 | nM | 8.41 |
| CHEMBL293263 | — | IC50 | = | 4.1 | nM | 8.39 |
| CHEMBL296373 | EDATREXATE | IC50 | = | 4.4 | nM | 8.36 |
| CHEMBL307820 | — | IC50 | = | 5.3 | nM | 8.28 |
| CHEMBL308279 | — | IC50 | = | 5.8 | nM | 8.24 |
| CHEMBL73267 | — | IC50 | = | 5.9 | nM | 8.23 |
| CHEMBL65616 | — | IC50 | = | 6.4 | nM | 8.19 |
| CHEMBL307486 | — | IC50 | = | 8.8 | nM | 8.06 |