Assay Detail
Binding
CHEMBL668433
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of dihydrofolate reductase(DHFR) enzyme from human leukemic lymphoblasts.
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Synthesis and in vitro biological activity of new deaza analogues of folic acid, aminopterin, and methotrexate with an L-ornithine side chain.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.53 | 7.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL35921 | — | — | 1 | 7.80 |
| CHEMBL32439 | — | — | 1 | 7.46 |
| CHEMBL32691 | — | — | 1 | 7.30 |
| CHEMBL280864 | — | — | 1 | 7.14 |
| CHEMBL36083 | — | — | 1 | 6.80 |
| CHEMBL35807 | — | — | 1 | 6.12 |
| CHEMBL35231 | — | — | 1 | 5.07 |
| CHEMBL287532 | — | — | 1 | 4.57 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL35921 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL32439 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL32691 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL280864 | — | IC50 | = | 72.0 | nM | 7.14 |
| CHEMBL36083 | — | IC50 | = | 160.0 | nM | 6.80 |
| CHEMBL35807 | — | IC50 | = | 750.0 | nM | 6.12 |
| CHEMBL35231 | — | IC50 | = | 8500.0 | nM | 5.07 |
| CHEMBL287532 | — | IC50 | = | 27000.0 | nM | 4.57 |