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Assay Detail

CHEMBL668436

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against Dihydrofolate reductase from recombinant human (hDHFR)
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents.
Gangjee A, Vidwans AP, Vasudevan A, Queener SF, Kisliuk RL, Cody V, Li R, Galitsky N, Luft JR, Pangborn W.
J Med Chem (1998) 41 : 3426 -3434
PubMed 9719595 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.75 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL113816 1 7.96
CHEMBL325434 1 7.58
CHEMBL114354 1 7.57
CHEMBL115804 1 7.30
CHEMBL22 TRIMETHOPRIM 4.0 1 7.27
CHEMBL117750 1 6.85
CHEMBL114839 1 6.22
CHEMBL114833 1 5.85
CHEMBL115474 1 5.62
CHEMBL36245 1 5.54
CHEMBL32113 1 5.07
CHEMBL32708 1 4.89
CHEMBL113999 1 4.64
CHEMBL114058 1 4.60
CHEMBL116200 1 4.58
CHEMBL116637 1 4.57
CHEMBL116815 1 4.55
CHEMBL116816 1 4.55
CHEMBL33208 1 4.12
CHEMBL119 TRIMETREXATE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL113816 IC50 = 11.0 nM 7.96
CHEMBL325434 IC50 = 26.0 nM 7.58
CHEMBL114354 IC50 = 27.0 nM 7.57
CHEMBL115804 IC50 = 50.0 nM 7.30
CHEMBL22 TRIMETHOPRIM IC50 = 54.0 nM 7.27
CHEMBL117750 IC50 = 140.0 nM 6.85
CHEMBL114839 IC50 = 600.0 nM 6.22
CHEMBL114833 IC50 = 1400.0 nM 5.85
CHEMBL115474 IC50 = 2400.0 nM 5.62
CHEMBL36245 IC50 = 2900.0 nM 5.54
CHEMBL32113 IC50 = 8500.0 nM 5.07
CHEMBL32708 IC50 = 13000.0 nM 4.89
CHEMBL113999 IC50 = 23000.0 nM 4.64
CHEMBL114058 IC50 = 25000.0 nM 4.60
CHEMBL116200 IC50 = 26000.0 nM 4.58
CHEMBL116637 IC50 = 27000.0 nM 4.57
CHEMBL116815 IC50 = 28000.0 nM 4.55
CHEMBL116816 IC50 = 28000.0 nM 4.55
CHEMBL33208 IC50 = 76000.0 nM 4.12
CHEMBL119 TRIMETREXATE IC50 = 340000.0 nM -