Assay Detail
Binding
CHEMBL668914
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was tested for its inhibitory concentration to inhibit the enzyme Dihydro Folate Reductase (DHFR) from murine L1210 leukemia cells.
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | L1210 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Methotrexate analogues. 28. Synthesis and biological evaluation of new gamma-monoamides of aminopterin and methotrexate.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 7.28 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.70 |
| CHEMBL376180 | AMINOPTERIN | 2.0 | 1 | 7.70 |
| CHEMBL44693 | — | — | 1 | 7.38 |
| CHEMBL44697 | — | — | 1 | 7.36 |
| CHEMBL47316 | — | — | 1 | 7.35 |
| CHEMBL45735 | — | — | 1 | 7.22 |
| CHEMBL48044 | — | — | 1 | 7.10 |
| CHEMBL46140 | — | — | 1 | 7.06 |
| CHEMBL45572 | — | — | 1 | 7.00 |
| CHEMBL416261 | — | — | 1 | 6.89 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL376180 | AMINOPTERIN | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL44693 | — | IC50 | = | 42.0 | nM | 7.38 |
| CHEMBL44697 | — | IC50 | = | 44.0 | nM | 7.36 |
| CHEMBL47316 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL45735 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL48044 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL46140 | — | IC50 | = | 87.0 | nM | 7.06 |
| CHEMBL45572 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL416261 | — | IC50 | = | 130.0 | nM | 6.89 |