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Assay Detail

CHEMBL669263

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Binding
Apparent dissociation constant at pH 6.6 for human dihydrofolate reductase in the presence of cofactor NADPH
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationships and pH dependence of binding of 8-alkyl-N5-deazapterins to dihydrofolate reductase.
Ivery MT, Gready JE.
J Med Chem (1994) 37 : 4211 -4221
PubMed 7990119 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 16 5.62 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL135937 1 7.10
CHEMBL436184 1 6.30
CHEMBL336628 1 6.22
CHEMBL135936 1 6.16
CHEMBL134613 1 6.16
CHEMBL340269 1 5.92
CHEMBL133360 1 5.72
CHEMBL135581 1 5.70
CHEMBL341200 1 5.54
CHEMBL135811 1 5.40
CHEMBL135905 1 5.34
CHEMBL424647 1 5.29
CHEMBL134466 1 4.89
CHEMBL134383 1 4.80
CHEMBL136042 1 4.75
CHEMBL135622 1 4.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL135937 Kd = 80.0 nM 7.10
CHEMBL436184 Kd = 500.0 nM 6.30
CHEMBL336628 Kd = 600.0 nM 6.22
CHEMBL135936 Kd = 700.0 nM 6.16
CHEMBL134613 Kd = 700.0 nM 6.16
CHEMBL340269 Kd = 1200.0 nM 5.92
CHEMBL133360 Kd = 1900.0 nM 5.72
CHEMBL135581 Kd = 2000.0 nM 5.70
CHEMBL341200 Kd = 2900.0 nM 5.54
CHEMBL135811 Kd = 4000.0 nM 5.40
CHEMBL135905 Kd = 4600.0 nM 5.34
CHEMBL424647 Kd = 5100.0 nM 5.29
CHEMBL134466 Kd = 13000.0 nM 4.89
CHEMBL134383 Kd = 16000.0 nM 4.80
CHEMBL136042 Kd = 18000.0 nM 4.75
CHEMBL135622 Kd = 21000.0 nM 4.68