Assay Detail
Binding
CHEMBL669305
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibitory activity towards dihydrofolate reductase in L1210 murine leukemia cell lines
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | L1210 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Methotrexate analogues. 23. Synthesis, dihydrofolate reductase affinity, cytotoxicity, and in vivo antitumor activity of some putative degradation products of methotrexate-poly(L-lysine) conjugates.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.07 | 7.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.30 |
| CHEMBL344876 | — | — | 1 | 7.07 |
| CHEMBL171937 | — | — | 1 | 7.06 |
| CHEMBL3143620 | — | — | 1 | 6.85 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL344876 | — | IC50 | = | 86.0 | nM | 7.07 |
| CHEMBL171937 | — | IC50 | = | 87.0 | nM | 7.06 |
| CHEMBL3143620 | — | IC50 | = | 140.0 | nM | 6.85 |