Assay Detail
Binding
CHEMBL669306
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Tested for inhibition against purified Dihydrofolate reductase from L1210 murine leukemia cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | L1210 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Methotrexate analogues. 34. Replacement of the glutamate moiety in methotrexate and aminopterin by long-chain 2-aminoalkanedioic acids.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 7.35 | 7.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL283062 | — | — | 1 | 7.64 |
| CHEMBL376180 | AMINOPTERIN | 2.0 | 1 | 7.60 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.60 |
| CHEMBL26326 | — | — | 1 | 7.58 |
| CHEMBL28112 | — | — | 1 | 7.54 |
| CHEMBL25923 | — | — | 1 | 7.50 |
| CHEMBL27306 | — | — | 1 | 7.47 |
| CHEMBL275229 | — | — | 1 | 7.17 |
| CHEMBL26148 | — | — | 1 | 7.09 |
| CHEMBL434004 | — | — | 1 | 6.27 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL283062 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL376180 | AMINOPTERIN | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL26326 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL28112 | — | IC50 | = | 29.0 | nM | 7.54 |
| CHEMBL25923 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL27306 | — | IC50 | = | 34.0 | nM | 7.47 |
| CHEMBL275229 | — | IC50 | = | 67.0 | nM | 7.17 |
| CHEMBL26148 | — | IC50 | = | 81.0 | nM | 7.09 |
| CHEMBL434004 | — | IC50 | = | 540.0 | nM | 6.27 |