Assay Detail
Binding
CHEMBL669442
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of dihydrofolate reductase in HeLa cells
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | HeLa |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Novel fluorinated antifolates. Enzyme inhibition and cytotoxicity studies on 2'- and 3'-fluoroaminopterin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.60 | 7.72 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL18925 | — | — | 1 | 7.72 |
| CHEMBL19227 | — | — | 1 | 7.57 |
| CHEMBL376180 | AMINOPTERIN | 2.0 | 1 | 7.51 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL18925 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL19227 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL376180 | AMINOPTERIN | IC50 | = | 31.0 | nM | 7.51 |