Assay Detail
Binding
CHEMBL669444
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human WIL2/dihydrofolate reductase activity
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Comparison of the biological effects of selected 5,8-dideazafolate analogues with their 2-desamino counterparts.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 6.56 | 8.37 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 8.37 |
| CHEMBL36739 | — | — | 1 | 6.96 |
| CHEMBL139551 | — | — | 1 | 6.77 |
| CHEMBL354121 | — | — | 1 | 6.58 |
| CHEMBL169376 | — | — | 1 | 6.50 |
| CHEMBL138870 | — | — | 1 | 6.48 |
| CHEMBL171790 | — | — | 1 | 6.19 |
| CHEMBL422395 | — | — | 1 | 6.04 |
| CHEMBL171480 | — | — | 1 | 5.16 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 4.3 | nM | 8.37 |
| CHEMBL36739 | — | IC50 | = | 110.0 | nM | 6.96 |
| CHEMBL139551 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL354121 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL169376 | — | IC50 | = | 320.0 | nM | 6.50 |
| CHEMBL138870 | — | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL171790 | — | IC50 | = | 650.0 | nM | 6.19 |
| CHEMBL422395 | — | IC50 | = | 910.0 | nM | 6.04 |
| CHEMBL171480 | — | IC50 | = | 7000.0 | nM | 5.16 |