Assay Detail
Binding
CHEMBL669631
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of [3H]- ouabain binding to digitalis receptor
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Cavia porcellus |
| Confidence | 4 — Cell-line / Tissue |
| Curated By | Autocuration |
Target
Sodium/potassium-transporting ATPase (CHEMBL2095186) ↗| Type | PROTEIN COMPLEX GROUP |
| Organism | Homo sapiens |
Publication
Synthesis of 20-hydroxy-, 20-amino-, and 20-nitro-14-hydroxy-21-nor-5 beta,14 beta-pregnane C-3 glycosides and related derivatives: structure-activity relationships of pregnanes that bind to the digitalis receptor.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 12 | 6.67 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1453 | DIGITOXIGENIN | — | 1 | 8.10 |
| CHEMBL254219 | DIGITOXIN | 4.0 | 1 | 8.10 |
| CHEMBL2069010 | — | — | 1 | 7.92 |
| CHEMBL2069112 | — | — | 1 | 7.35 |
| CHEMBL2068949 | — | — | 1 | 7.06 |
| CHEMBL2069062 | — | — | 1 | 7.00 |
| CHEMBL2069127 | — | — | 1 | 6.57 |
| CHEMBL2069005 | — | — | 1 | 6.44 |
| CHEMBL2068948 | — | — | 1 | 6.21 |
| CHEMBL2068950 | — | — | 1 | 5.97 |
| CHEMBL2069045 | — | — | 1 | 4.82 |
| CHEMBL2069011 | — | — | 1 | 4.44 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1453 | DIGITOXIGENIN | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL254219 | DIGITOXIN | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL2069010 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL2069112 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL2068949 | — | IC50 | = | 88.0 | nM | 7.06 |
| CHEMBL2069062 | — | IC50 | = | 99.0 | nM | 7.00 |
| CHEMBL2069127 | — | IC50 | = | 270.0 | nM | 6.57 |
| CHEMBL2069005 | — | IC50 | = | 360.0 | nM | 6.44 |
| CHEMBL2068948 | — | IC50 | = | 610.0 | nM | 6.21 |
| CHEMBL2068950 | — | IC50 | = | 1070.0 | nM | 5.97 |
| CHEMBL2069045 | — | IC50 | = | 15000.0 | nM | 4.82 |
| CHEMBL2069011 | — | IC50 | = | 36000.0 | nM | 4.44 |