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Assay Detail

CHEMBL669642

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Binding
Evaluated for the inhibition of dihydrofolate reductase from L1210 mouse leukemia cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL4564)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Chemistry and antitumor evaluation of selected classical 2,4-diaminoquinazoline analogues of folic acid.
Hynes JB, Harmon SJ, Floyd GG, Farrington M, Hart LD, Gale GR, Washtien WL, Susten SS, Freisheim JH.
J Med Chem (1985) 28 : 209 -215
PubMed 3968685 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.26 7.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 7.35
CHEMBL305253 1 7.29
CHEMBL423123 1 7.22
CHEMBL69713 1 7.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 45.0 nM 7.35
CHEMBL305253 IC50 = 51.0 nM 7.29
CHEMBL423123 IC50 = 60.0 nM 7.22
CHEMBL69713 IC50 = 70.0 nM 7.16