Assay Detail
Binding
CHEMBL669645
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was tested for the inhibition of dihydrofolate reductase in P388 cells
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | P388 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Synthesis and antitumor activities of novel 6-5 fused ring heterocycle antifolates: N-[4-[omega-(2-amino-4-substituted-6,7-dihydrocyclopenta [d]pyrimidin-5-yl)alkyl]benzoyl]-L-glutamic acids.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.89 | 8.68 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL296190 | — | — | 1 | 8.68 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 8.18 |
| CHEMBL41056 | — | — | 1 | 8.15 |
| CHEMBL296373 | EDATREXATE | 2.0 | 1 | 7.96 |
| CHEMBL42301 | — | — | 1 | 7.22 |
| CHEMBL42159 | — | — | 1 | 7.14 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL296190 | — | IC50 | = | 2.1 | nM | 8.68 |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 6.6 | nM | 8.18 |
| CHEMBL41056 | — | IC50 | = | 7.1 | nM | 8.15 |
| CHEMBL296373 | EDATREXATE | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL42301 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL42159 | — | IC50 | = | 72.0 | nM | 7.14 |