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Assay Detail

CHEMBL669646

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of dihydrofolate reductase (DHFR) from murine leukemia cells
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL4564)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Methotrexate analogues. 33. N delta-acyl-N alpha-(4-amino-4-deoxypteroyl)-L-ornithine derivatives: synthesis and in vitro antitumor activity.
Rosowsky A, Bader H, Cucchi CA, Moran RG, Kohler W, Freisheim JH.
J Med Chem (1988) 31 : 1332 -1337
PubMed 3385730 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.45 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL283702 1 7.82
CHEMBL34259 METHOTREXATE 4.0 1 7.60
CHEMBL27109 1 7.57
CHEMBL441418 1 7.55
CHEMBL423297 1 7.55
CHEMBL416193 1 7.50
CHEMBL27514 1 7.47
CHEMBL376180 AMINOPTERIN 2.0 1 7.46
CHEMBL27821 1 7.35
CHEMBL26534 1 7.28
CHEMBL18155 1 7.28
CHEMBL405237 1 7.25
CHEMBL280864 1 7.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL283702 IC50 = 15.0 nM 7.82
CHEMBL34259 METHOTREXATE IC50 = 25.0 nM 7.60
CHEMBL27109 IC50 = 27.0 nM 7.57
CHEMBL441418 IC50 = 28.0 nM 7.55
CHEMBL423297 IC50 = 28.0 nM 7.55
CHEMBL416193 IC50 = 32.0 nM 7.50
CHEMBL27514 IC50 = 34.0 nM 7.47
CHEMBL376180 AMINOPTERIN IC50 = 35.0 nM 7.46
CHEMBL27821 IC50 = 45.0 nM 7.35
CHEMBL26534 IC50 = 53.0 nM 7.28
CHEMBL18155 IC50 = 52.0 nM 7.28
CHEMBL405237 IC50 = 56.0 nM 7.25
CHEMBL280864 IC50 = 72.0 nM 7.14