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Assay Detail

CHEMBL669982

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory concentration against Dihydrofolate reductase from Mycobacterium avium (Mycobacterium avium)
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mycobacterium avium
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL5457)
Type SINGLE PROTEIN
Organism Mycobacterium avium

Publication

Design, synthesis, and biological evaluation of 2,4-diamino-5-methyl-6-substituted-pyrrolo[2,3-d]pyrimidines as dihydrofolate reductase inhibitors.
Gangjee A, Lin X, Queener SF.
J Med Chem (2004) 47 : 3689 -3692
PubMed 15214795 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.26 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL119 TRIMETREXATE 4.0 1 8.82
CHEMBL331986 1 7.00
CHEMBL331314 1 6.52
CHEMBL331292 1 6.52
CHEMBL123892 1 6.22
CHEMBL122998 1 6.16
CHEMBL123984 1 5.86
CHEMBL420172 1 5.40
CHEMBL334198 1 5.36
CHEMBL331456 1 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL119 TRIMETREXATE IC50 = 1.5 nM 8.82
CHEMBL331986 IC50 = 100.0 nM 7.00
CHEMBL331314 IC50 = 300.0 nM 6.52
CHEMBL331292 IC50 = 300.0 nM 6.52
CHEMBL123892 IC50 = 600.0 nM 6.22
CHEMBL122998 IC50 = 700.0 nM 6.16
CHEMBL123984 IC50 = 1370.0 nM 5.86
CHEMBL420172 IC50 = 4000.0 nM 5.40
CHEMBL334198 IC50 = 4400.0 nM 5.36
CHEMBL331456 IC50 = 19000.0 nM 4.72