Assay Detail
Binding
CHEMBL669988
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Compound was evaluated as inhibitor of human Dihydrofolate reductase
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Design, synthesis, and biological activities of classical N-[4-[2-(2-amino-4-ethylpyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-l-glutamic acid and its 6-methyl derivative as potential dual inhibitors of thymidylate synthase and dihydrofolate reductase and as potential antitumor agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 5.93 | 7.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.66 |
| CHEMBL157434 | — | — | 1 | 6.04 |
| CHEMBL439973 | — | — | 1 | 5.92 |
| CHEMBL225072 | PEMETREXED | 4.0 | 1 | 5.85 |
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | 5.47 |
| CHEMBL154935 | — | — | 1 | 4.66 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL157434 | — | IC50 | = | 920.0 | nM | 6.04 |
| CHEMBL439973 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL225072 | PEMETREXED | IC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL22 | TRIMETHOPRIM | IC50 | = | 3400.0 | nM | 5.47 |
| CHEMBL154935 | — | IC50 | = | 22000.0 | nM | 4.66 |