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Assay Detail

CHEMBL673949

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Binding
Inhibition of heregulin-stimulated autophosphorylation of ERBB2 receptor kinase in MDA-MB-453 cells.
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDA-MB-453
Confidence 9 — Direct single protein target
Curated By Expert

Target

Receptor tyrosine-protein kinase erbB-2 (CHEMBL1824)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tyrosine kinase inhibitors. 15. 4-(Phenylamino)quinazoline and 4-(phenylamino)pyrido[d]pyrimidine acrylamides as irreversible inhibitors of the ATP binding site of the epidermal growth factor receptor.
Smaill JB, Palmer BD, Rewcastle GW, Denny WA, McNamara DJ, Dobrusin EM, Bridges AJ, Zhou H, Showalter HD, Winters RT, Leopold WR, Fry DW, Nelson JM, Slintak V, Elliot WL, Roberts BJ, Vincent PW, Patmore SJ.
J Med Chem (1999) 42 : 1803 -1815
PubMed 10346932 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 8.18 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL54091 1 8.96
CHEMBL31815 1 8.66
CHEMBL53555 1 8.52
CHEMBL51659 1 8.41
CHEMBL31373 1 8.37
CHEMBL285063 1 8.24
CHEMBL280757 1 8.14
CHEMBL430571 1 8.12
CHEMBL53203 1 8.07
CHEMBL28418 1 7.92
CHEMBL53665 1 7.58
CHEMBL443523 1 7.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL54091 IC50 = 1.1 nM 8.96
CHEMBL31815 IC50 = 2.2 nM 8.66
CHEMBL53555 IC50 = 3.0 nM 8.52
CHEMBL51659 IC50 = 3.9 nM 8.41
CHEMBL31373 IC50 = 4.3 nM 8.37
CHEMBL285063 IC50 = 5.7 nM 8.24
CHEMBL280757 IC50 = 7.3 nM 8.14
CHEMBL430571 IC50 = 7.6 nM 8.12
CHEMBL53203 IC50 = 8.6 nM 8.07
CHEMBL28418 IC50 = 12.0 nM 7.92
CHEMBL53665 IC50 = 26.0 nM 7.58
CHEMBL443523 IC50 = 73.0 nM 7.14