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Assay Detail

CHEMBL676632

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Tested for binding affinity for human Endothelin B receptor by measuring its ability to displace [125I]ET-3 from chinese hamster ovary cells(CHO)
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type ovary
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Endothelin receptor type B (CHEMBL1785)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrrolidine-3-carboxylic acids as endothelin antagonists. 3. Discovery of a potent, 2-nonaryl, highly selective ETA antagonist (A-216546).
Liu G, Henry KJ, Szczepankiewicz BG, Winn M, Kozmina NS, Boyd SA, Wasicak J, von Geldern TW, Wu-Wong JR, Chiou WJ, Dixon DB, Nguyen B, Marsh KC, Opgenorth TJ.
J Med Chem (1998) 41 : 3261 -3275
PubMed 9703472 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 4.63 5.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL432974 1 5.55
CHEMBL111612 ABT-546 1 4.81
CHEMBL110053 1 4.75
CHEMBL109778 1 4.50
CHEMBL109926 1 4.40
CHEMBL322537 1 4.38
CHEMBL323951 1 4.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL432974 IC50 = 2800.0 nM 5.55
CHEMBL111612 ABT-546 IC50 = 15400.0 nM 4.81
CHEMBL110053 IC50 = 18000.0 nM 4.75
CHEMBL109778 IC50 = 32000.0 nM 4.50
CHEMBL109926 IC50 = 39700.0 nM 4.40
CHEMBL322537 IC50 = 42000.0 nM 4.38
CHEMBL323951 IC50 = 92700.0 nM 4.03