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Assay Detail

CHEMBL681286

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Concentration required to displace 50% of a radiolabeled farnesyltransferase inhibitor from FPTase in cultured Ha-Ras transformed RAT1 cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type Rat1
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Protein farnesyltransferase (CHEMBL2094108)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Aryloxy substituted N-arylpiperazinones as dual inhibitors of farnesyltransferase and geranylgeranyltransferase-I.
Bergman JM, Abrams MT, Davide JP, Greenberg IB, Robinson RG, Buser CA, Huber HE, Koblan KS, Kohl NE, Lobell RB, Graham SL, Hartman GD, Williams TM, Dinsmore CJ.
Bioorg Med Chem Lett (2001) 11 : 1411 -1415
PubMed 11378366 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.27 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL279433 L-778123 FREE BASE 1.0 1 8.52
CHEMBL283203 1 8.10
CHEMBL30270 1 7.77
CHEMBL418036 1 7.39
CHEMBL31828 1 7.32
CHEMBL30349 1 7.03
CHEMBL31031 1 6.96
CHEMBL439917 1 6.59
CHEMBL30858 1 6.51
CHEMBL30925 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL279433 L-778123 FREE BASE IC50 = 3.0 nM 8.52
CHEMBL283203 IC50 = 8.0 nM 8.10
CHEMBL30270 IC50 = 17.0 nM 7.77
CHEMBL418036 IC50 = 41.0 nM 7.39
CHEMBL31828 IC50 = 48.0 nM 7.32
CHEMBL30349 IC50 = 93.0 nM 7.03
CHEMBL31031 IC50 = 110.0 nM 6.96
CHEMBL439917 IC50 = 255.0 nM 6.59
CHEMBL30858 IC50 = 311.0 nM 6.51
CHEMBL30925 IC50 = 350.0 nM 6.46