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Assay Detail

CHEMBL682252

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity by displacement of [3H]Ro-151788 from recombinant human gamma-aminobutyric-acid A receptor alpha3,beta3,gamma2 stably expressed in L cells
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type L
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-3/beta-3/gamma-2 (CHEMBL2094120)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

3-Heteroaryl-2-pyridones: benzodiazepine site ligands with functional delectivity for alpha 2/alpha 3-subtypes of human GABA(A) receptor-ion channels.
Collins I, Moyes C, Davey WB, Rowley M, Bromidge FA, Quirk K, Atack JR, McKernan RM, Thompson SA, Wafford K, Dawson GR, Pike A, Sohal B, Tsou NN, Ball RG, Castro JL.
J Med Chem (2002) 45 : 1887 -1900
PubMed 11960500 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 28 7.40 8.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL294600 1 8.47
CHEMBL55565 1 8.46
CHEMBL264235 1 8.42
CHEMBL17468 1 8.40
CHEMBL56098 1 8.39
CHEMBL59478 1 8.21
CHEMBL59427 1 8.17
CHEMBL54422 1 7.92
CHEMBL59197 1 7.92
CHEMBL55022 1 7.82
CHEMBL54368 1 7.64
CHEMBL53619 1 7.62
CHEMBL56761 1 7.60
CHEMBL55570 1 7.22
CHEMBL291335 1 7.14
CHEMBL413325 1 7.10
CHEMBL301195 1 6.68
CHEMBL56746 1 6.60
CHEMBL299061 1 6.60
CHEMBL298732 1 6.57
CHEMBL58927 1 6.57
CHEMBL451 CHLORDIAZEPOXIDE 4.0 1 6.56
CHEMBL55467 1 6.50
CHEMBL54507 1 6.29
CHEMBL301010 1 6.07
CHEMBL291308 1 -
CHEMBL55777 1 -
CHEMBL55694 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL294600 Ki = 3.4 nM 8.47
CHEMBL55565 Ki = 3.5 nM 8.46
CHEMBL264235 Ki = 3.8 nM 8.42
CHEMBL17468 Ki = 4.0 nM 8.40
CHEMBL56098 Ki = 4.1 nM 8.39
CHEMBL59478 Ki = 6.2 nM 8.21
CHEMBL59427 Ki = 6.7 nM 8.17
CHEMBL54422 Ki = 12.0 nM 7.92
CHEMBL59197 Ki = 12.0 nM 7.92
CHEMBL55022 Ki = 15.0 nM 7.82
CHEMBL54368 Ki = 23.0 nM 7.64
CHEMBL53619 Ki = 24.0 nM 7.62
CHEMBL56761 Ki = 25.0 nM 7.60
CHEMBL55570 Ki = 60.0 nM 7.22
CHEMBL291335 Ki = 73.0 nM 7.14
CHEMBL413325 Ki = 79.0 nM 7.10
CHEMBL301195 Ki = 210.0 nM 6.68
CHEMBL56746 Ki = 250.0 nM 6.60
CHEMBL299061 Ki = 250.0 nM 6.60
CHEMBL298732 Ki = 270.0 nM 6.57
CHEMBL58927 Ki = 270.0 nM 6.57
CHEMBL451 CHLORDIAZEPOXIDE Ki = 275.0 nM 6.56
CHEMBL55467 Ki = 320.0 nM 6.50
CHEMBL54507 Ki = 510.0 nM 6.29
CHEMBL301010 Ki = 860.0 nM 6.07
CHEMBL291308 Ki > 30000.0 nM -
CHEMBL55777 Ki > 1000.0 nM -
CHEMBL55694 Ki > 30000.0 nM -