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Assay Detail

CHEMBL684724

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human Glycinamide Ribonucleotide Transformylase (GART) using N10-formyl-5,8-dideazafolate (FDDF) as the cofactor.
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Trifunctional purine biosynthetic protein adenosine-3 (CHEMBL3972)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Protein structure-based design, synthesis, and biological evaluation of 5-thia-2,6-diamino-4(3H)-oxopyrimidines: potent inhibitors of glycinamide ribonucleotide transformylase with potent cell growth inhibition.
Varney MD, Palmer CL, Romines WH, Boritzki T, Margosiak SA, Almassy R, Janson CA, Bartlett C, Howland EJ, Ferre R.
J Med Chem (1997) 40 : 2502 -2524
PubMed 9258357 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.38 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL82261 1 8.70
CHEMBL85436 1 8.52
CHEMBL82181 1 8.47
CHEMBL84935 1 8.07
CHEMBL84605 1 7.85
CHEMBL82390 1 7.82
CHEMBL84163 1 7.55
CHEMBL309415 1 7.52
CHEMBL315627 1 7.50
CHEMBL85871 1 7.46
CHEMBL315076 1 7.46
CHEMBL84904 1 7.42
CHEMBL84045 1 6.08
CHEMBL85848 1 5.70
CHEMBL84728 1 4.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL82261 Ki = 2.0 nM 8.70
CHEMBL85436 Ki = 3.0 nM 8.52
CHEMBL82181 Ki = 3.4 nM 8.47
CHEMBL84935 Ki = 8.5 nM 8.07
CHEMBL84605 Ki = 14.0 nM 7.85
CHEMBL82390 Ki = 15.0 nM 7.82
CHEMBL84163 Ki = 28.0 nM 7.55
CHEMBL309415 Ki = 30.0 nM 7.52
CHEMBL315627 Ki = 32.0 nM 7.50
CHEMBL85871 Ki = 35.0 nM 7.46
CHEMBL315076 Ki = 35.0 nM 7.46
CHEMBL84904 Ki = 38.0 nM 7.42
CHEMBL84045 Ki = 825.0 nM 6.08
CHEMBL85848 Ki = 2000.0 nM 5.70
CHEMBL84728 Ki = 30000.0 nM 4.52