Assay Detail
Functional
CHEMBL688480
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Growth inhibition of H35R0.3 cell lines
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | H35R0.3 |
| Confidence | 1 — Organism |
| Curated By | Intermediate |
Publication
Methotrexate analogues. 23. Synthesis, dihydrofolate reductase affinity, cytotoxicity, and in vivo antitumor activity of some putative degradation products of methotrexate-poly(L-lysine) conjugates.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.23 | 5.75 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 5.75 |
| CHEMBL171937 | — | — | 1 | 5.22 |
| CHEMBL344876 | — | — | 1 | 5.02 |
| CHEMBL3143620 | — | — | 1 | 4.92 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 1800.0 | nM | 5.75 |
| CHEMBL171937 | — | IC50 | = | 6000.0 | nM | 5.22 |
| CHEMBL344876 | — | IC50 | = | 9500.0 | nM | 5.02 |
| CHEMBL3143620 | — | IC50 | = | 12000.0 | nM | 4.92 |