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Assay Detail

CHEMBL688484

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Compound was tested for its inhibitory activity in tumor cell growth in H35R cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type H35R0.3
Confidence 1 — Organism
Curated By Intermediate

Target

H35R0.3 (CHEMBL614160)
Type CELL-LINE
Organism Rattus norvegicus

Publication

Folate analogues. 34. Synthesis and antitumor activity of non-polyglutamylatable inhibitors of dihydrofolate reductase.
Abraham A, McGuire JJ, Galivan J, Nimec Z, Kisliuk RL, Gaumont Y, Nair MG.
J Med Chem (1991) 34 : 222 -227
PubMed 1992121 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.88 5.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL307820 1 5.25
CHEMBL308279 1 4.85
CHEMBL307486 1 4.74
CHEMBL65616 1 4.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL307820 IC50 = 5600.0 nM 5.25
CHEMBL308279 IC50 = 14000.0 nM 4.85
CHEMBL307486 IC50 = 18400.0 nM 4.74
CHEMBL65616 IC50 = 21000.0 nM 4.68