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Assay Detail

CHEMBL690460

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxic potency required to inhibit HT-29 cell growth by 50% after cell drug contact for 144 hrs
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Expert

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

1-[(omega-aminoalkyl)amino]-4-[N-(omega-aminoalkyl)carbamoyl]-9-oxo-9, 10-dihydroacridines as intercalating cytotoxic agents: synthesis, DNA binding, and biological evaluation.
Antonini I, Polucci P, Jenkins TC, Kelland LR, Menta E, Pescalli N, Stefanska B, Mazerski J, Martelli S.
J Med Chem (1997) 40 : 3749 -3755
PubMed 9371240 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.16 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL58 MITOXANTRONE 4.0 1 8.00
CHEMBL104900 1 7.03
CHEMBL327096 1 6.72
CHEMBL125371 1 6.35
CHEMBL105362 1 6.30
CHEMBL123024 1 6.24
CHEMBL104004 1 5.70
CHEMBL126010 1 5.52
CHEMBL340343 1 5.35
CHEMBL124605 1 5.35
CHEMBL125180 1 5.24
CHEMBL121823 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL58 MITOXANTRONE IC50 = 10.0 nM 8.00
CHEMBL104900 IC50 = 94.0 nM 7.03
CHEMBL327096 IC50 = 190.0 nM 6.72
CHEMBL125371 IC50 = 450.0 nM 6.35
CHEMBL105362 IC50 = 500.0 nM 6.30
CHEMBL123024 IC50 = 580.0 nM 6.24
CHEMBL104004 IC50 = 2000.0 nM 5.70
CHEMBL126010 IC50 = 3000.0 nM 5.52
CHEMBL340343 IC50 = 4500.0 nM 5.35
CHEMBL124605 IC50 = 4500.0 nM 5.35
CHEMBL125180 IC50 = 5700.0 nM 5.24
CHEMBL121823 IC50 > 23000.0 nM -