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Assay Detail

CHEMBL690470

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of cell growth against HT-29 human gastrointestinal adenocarcinoma cell lines in vitro
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Intermediate

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Synthesis of 5-chloro-5,8-dideaza analogues of folic acid and aminopterin targeted for colon adenocarcinoma.
Hynes JB, Kumar A, Tomazic A, Washtien WL.
J Med Chem (1987) 30 : 1515 -1519
PubMed 3612694 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.27 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL37061 1 8.64
CHEMBL36323 1 8.52
CHEMBL34259 METHOTREXATE 4.0 1 7.75
CHEMBL39674 1 7.60
CHEMBL36866 1 7.22
CHEMBL35936 1 5.96
CHEMBL39851 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL37061 IC50 = 2.3 nM 8.64
CHEMBL36323 IC50 = 3.0 nM 8.52
CHEMBL34259 METHOTREXATE IC50 = 18.0 nM 7.75
CHEMBL39674 IC50 = 25.0 nM 7.60
CHEMBL36866 IC50 = 60.0 nM 7.22
CHEMBL35936 IC50 = 1100.0 nM 5.96
CHEMBL39851 IC50 = 6700.0 nM 5.17