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Assay Detail

CHEMBL690540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Compound was tested for inhibition of folinic acid transport in H35 Hepatoma cells.
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type H35R0.3
Confidence 1 — Organism
Curated By Intermediate

Target

H35R0.3 (CHEMBL614160)
Type CELL-LINE
Organism Rattus norvegicus

Publication

Folate analogues. 34. Synthesis and antitumor activity of non-polyglutamylatable inhibitors of dihydrofolate reductase.
Abraham A, McGuire JJ, Galivan J, Nimec Z, Kisliuk RL, Gaumont Y, Nair MG.
J Med Chem (1991) 34 : 222 -227
PubMed 1992121 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.25 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL308279 1 5.82
CHEMBL307486 1 5.26
CHEMBL65616 1 5.16
CHEMBL34259 METHOTREXATE 4.0 1 4.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL308279 IC50 = 1500.0 nM 5.82
CHEMBL307486 IC50 = 5500.0 nM 5.26
CHEMBL65616 IC50 = 7000.0 nM 5.16
CHEMBL34259 METHOTREXATE IC50 = 18000.0 nM 4.75