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Assay Detail

CHEMBL690542

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Functional
Growth inhibition of H35 cell lines
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type H35
Confidence 1 — Organism
Curated By Expert

Target

H35 (CHEMBL614784)
Type CELL-LINE
Organism Rattus norvegicus

Publication

Methotrexate analogues. 23. Synthesis, dihydrofolate reductase affinity, cytotoxicity, and in vivo antitumor activity of some putative degradation products of methotrexate-poly(L-lysine) conjugates.
Rosowsky A, Forsch RA, Freisheim JH, Galivan J, Wick M.
J Med Chem (1984) 27 : 888 -893
PubMed 6737432 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.74 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 8.00
CHEMBL171937 1 6.40
CHEMBL344876 1 6.30
CHEMBL3143620 1 6.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 10.0 nM 8.00
CHEMBL171937 IC50 = 400.0 nM 6.40
CHEMBL344876 IC50 = 500.0 nM 6.30
CHEMBL3143620 IC50 = 560.0 nM 6.25