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Assay Detail

CHEMBL692391

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Human tumor cell cytotoxicity assay was performed using MTT (HT-29 cell line )
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Intermediate

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Water soluble inhibitors of topoisomerase I: quaternary salt derivatives of camptothecin.
Lackey K, Sternbach DD, Croom DK, Emerson DL, Evans MG, Leitner PL, Luzzio MJ, McIntyre G, Vuong A, Yates J, Besterman JM.
J Med Chem (1996) 39 : 713 -719
PubMed 8576914 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.31 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL422886 1 7.89
CHEMBL350118 1 7.80
CHEMBL84 TOPOTECAN 4.0 1 7.60
CHEMBL445914 1 7.34
CHEMBL162904 1 7.16
CHEMBL163006 1 7.12
CHEMBL423808 1 7.08
CHEMBL53463 DOXORUBICIN 4.0 1 6.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL422886 IC50 = 13.0 nM 7.89
CHEMBL350118 IC50 = 16.0 nM 7.80
CHEMBL84 TOPOTECAN IC50 = 25.0 nM 7.60
CHEMBL445914 IC50 = 46.0 nM 7.34
CHEMBL162904 IC50 = 70.0 nM 7.16
CHEMBL163006 IC50 = 75.0 nM 7.12
CHEMBL423808 IC50 = 84.0 nM 7.08
CHEMBL53463 DOXORUBICIN IC50 = 330.0 nM 6.48