Assay Detail
Binding
CHEMBL694305
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of histidine protein kinase (KinA) phosphorylation in the presence of response regulator (Spo0F)
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 4 — Cell-line / Tissue |
| Curated By | Expert |
Target
KinA/Spo0F (sporulation kinase A/sporulation initiation phosphotransferase F) (CHEMBL2111331) ↗| Type | PROTEIN FAMILY |
| Organism | Bacillus subtilis (strain 168) |
Publication
Novel inhibitors of bacterial two-component systems with gram positive antibacterial activity: pharmacophore identification based on the screening hit closantel.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 14 | 4.80 | 5.42 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL57324 | — | — | 1 | 5.42 |
| CHEMBL56579 | — | — | 1 | 5.32 |
| CHEMBL57687 | — | — | 1 | 5.05 |
| CHEMBL57656 | — | — | 1 | 4.66 |
| CHEMBL291338 | 3,3',4',5-TETRACHLOROSALICYLANILIDE | 4.0 | 1 | 4.35 |
| CHEMBL56781 | — | — | 1 | 4.01 |
| CHEMBL57828 | — | — | 1 | - |
| CHEMBL57526 | — | — | 1 | - |
| CHEMBL293285 | — | — | 1 | - |
| CHEMBL57924 | — | — | 1 | - |
| CHEMBL294403 | — | — | 1 | - |
| CHEMBL301903 | — | — | 1 | - |
| CHEMBL299692 | — | — | 1 | - |
| CHEMBL57827 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL57324 | — | IC50 | = | 3800.0 | nM | 5.42 |
| CHEMBL56579 | — | IC50 | = | 4800.0 | nM | 5.32 |
| CHEMBL57687 | — | IC50 | = | 9000.0 | nM | 5.05 |
| CHEMBL57656 | — | IC50 | = | 22000.0 | nM | 4.66 |
| CHEMBL291338 | 3,3',4',5-TETRACHLOROSALICYLANILIDE | IC50 | = | 45000.0 | nM | 4.35 |
| CHEMBL56781 | — | IC50 | = | 98000.0 | nM | 4.01 |
| CHEMBL57828 | — | IC50 | > | 500000.0 | nM | - |
| CHEMBL57526 | — | IC50 | > | 500000.0 | nM | - |
| CHEMBL293285 | — | IC50 | > | 500000.0 | nM | - |
| CHEMBL57924 | — | IC50 | > | 500000.0 | nM | - |
| CHEMBL294403 | — | IC50 | > | 500000.0 | nM | - |
| CHEMBL301903 | — | IC50 | > | 500000.0 | nM | - |
| CHEMBL299692 | — | IC50 | = | 385000.0 | nM | - |
| CHEMBL57827 | — | IC50 | = | 365000.0 | nM | - |