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Assay Detail

CHEMBL695208

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro cytotoxic activity against colon adenocarcinoma (HT-29) cells assayed by inhibition of [3H]-labeled thymidine incorporation
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Intermediate

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

O-Phosphonatomethylcholine, its analogues, alkyl esters, and their biological activity.
Holý A, Otová B, Budesínský M, Emerson D, Wiles ME.
J Med Chem (2001) 44 : 4462 -4467
PubMed 11728191 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.46 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL90555 VINCRISTINE 4.0 1 11.00
CHEMBL428647 PACLITAXEL 4.0 1 9.80
CHEMBL888 GEMCITABINE 4.0 1 8.52
CHEMBL138287 1 5.32
CHEMBL125 MILTEFOSINE 4.0 1 5.21
CHEMBL140357 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL90555 VINCRISTINE IC50 = 0.01 nM 11.00
CHEMBL428647 PACLITAXEL IC50 = 0.16 nM 9.80
CHEMBL888 GEMCITABINE IC50 = 3.0 nM 8.52
CHEMBL138287 IC50 = 4755.0 nM 5.32
CHEMBL125 MILTEFOSINE IC50 = 6150.0 nM 5.21
CHEMBL140357 IC50 = 12170.0 nM 4.92