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Assay Detail

CHEMBL696446

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Binding
Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type SK-N-MC
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Histamine H4 receptor (CHEMBL3759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of conformationally constrained histamine H(3) receptor agonists.
Kitbunnadaj R, Zuiderveld OP, De Esch IJ, Vollinga RC, Bakker R, Lutz M, Spek AL, Cavoy E, Deltent MF, Menge WM, Timmerman H, Leurs R.
J Med Chem (2003) 46 : 5445 -5457
PubMed 14640553 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 26 6.24 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL90 HISTAMINE 4.0 1 7.26
CHEMBL18661 IMMEPIP 1 7.25
CHEMBL90019 IMBUTAMINE 1 7.05
CHEMBL91353 HOMOHISTAMINE 1 6.67
CHEMBL440730 1 6.38
CHEMBL153061 1 6.13
CHEMBL153051 1 5.99
CHEMBL153284 1 5.96
CHEMBL268229 1 5.95
CHEMBL153207 1 5.69
CHEMBL151467 1 5.40
CHEMBL13795 VUF-5297 1 5.15
CHEMBL152936 1 -
CHEMBL150701 1 -
CHEMBL13559 VUF-5296 1 -
CHEMBL154529 1 -
CHEMBL152948 1 -
CHEMBL356666 1 -
CHEMBL345954 1 -
CHEMBL417096 IMPENTAMINE 1 -
CHEMBL150703 1 -
CHEMBL150243 1 -
CHEMBL358028 1 -
CHEMBL150512 1 -
CHEMBL554031 1 -
CHEMBL78498 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL90 HISTAMINE EC50 = 54.95 nM 7.26
CHEMBL18661 IMMEPIP EC50 = 56.23 nM 7.25
CHEMBL90019 IMBUTAMINE EC50 = 89.13 nM 7.05
CHEMBL91353 HOMOHISTAMINE EC50 = 213.8 nM 6.67
CHEMBL440730 EC50 = 416.87 nM 6.38
CHEMBL153061 EC50 = 741.31 nM 6.13
CHEMBL153051 EC50 = 1023.29 nM 5.99
CHEMBL153284 EC50 = 1096.48 nM 5.96
CHEMBL268229 EC50 = 1122.02 nM 5.95
CHEMBL153207 EC50 = 2041.74 nM 5.69
CHEMBL151467 EC50 = 3981.07 nM 5.40
CHEMBL13795 VUF-5297 EC50 = 7079.46 nM 5.15
CHEMBL152936 EC50 < 10000.0 nM -
CHEMBL150701 EC50 < 10000.0 nM -
CHEMBL13559 VUF-5296 EC50 < 10000.0 nM -
CHEMBL154529 EC50 < 10000.0 nM -
CHEMBL152948 EC50 < 10000.0 nM -
CHEMBL356666 EC50 < 10000.0 nM -
CHEMBL345954 EC50 < 10000.0 nM -
CHEMBL417096 IMPENTAMINE EC50 < 10000.0 nM -
CHEMBL150703 EC50 < 10000.0 nM -
CHEMBL150243 EC50 < 10000.0 nM -
CHEMBL358028 EC50 < 10000.0 nM -
CHEMBL150512 EC50 < 10000.0 nM -
CHEMBL554031 EC50 < 10000.0 nM -
CHEMBL78498 EC50 < 10000.0 nM -