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Assay Detail

CHEMBL696712

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibitory concentration required for cytotoxicity in J774.2 murine macrophage-like cells
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type J774
Confidence 1 — Organism
Curated By Intermediate

Target

J774 (CHEMBL614123)
Type CELL-LINE
Organism Mus musculus

Publication

Hydroxylation at C-3 of doxorubicin alters the selected phenotype of cellular drug resistance
Lothstein L, Sweatman TW, Priebe W
Bioorg Med Chem Lett (1995) 5 : 1807 -1812
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.13 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL159 VINBLASTINE 4.0 1 8.68
CHEMBL61 PODOFILOX 4.0 1 7.79
CHEMBL428647 PACLITAXEL 4.0 1 7.18
CHEMBL53463 DOXORUBICIN 4.0 1 7.10
CHEMBL107 COLCHICINE 4.0 1 7.03
CHEMBL432707 1 6.97
CHEMBL44657 ETOPOSIDE 4.0 1 6.24
CHEMBL43 AMSACRINE 4.0 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL159 VINBLASTINE IC50 = 2.1 nM 8.68
CHEMBL61 PODOFILOX IC50 = 16.4 nM 7.79
CHEMBL428647 PACLITAXEL IC50 = 65.6 nM 7.18
CHEMBL53463 DOXORUBICIN IC50 = 79.4 nM 7.10
CHEMBL107 COLCHICINE IC50 = 93.1 nM 7.03
CHEMBL432707 IC50 = 107.7 nM 6.97
CHEMBL44657 ETOPOSIDE IC50 = 576.7 nM 6.24
CHEMBL43 AMSACRINE IC50 = 900.6 nM 6.04