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Assay Detail

CHEMBL697364

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro binding affinity towards ionotropic glutamate receptor AMPA using [3H]AMPA as radioligand in rat cortical membrane preparations
27
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate receptor ionotropic, AMPA (CHEMBL2093871)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Spiro-imidazo[1,2-a]indeno[1,2-e]pyrazine-4-one derivatives are mixed AMPA and NMDA glycine-site antagonists active in vivo.
Jimonet P, Boireau A, Chevé M, Damour D, Genevois-Borella A, Imperato A, Pratt J, Randle JC, Ribeill Y, Stutzmann JM, Mignani S.
Bioorg Med Chem Lett (1999) 9 : 2921 -2926
PubMed 10571148 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 5.93 7.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL14712 3 7.07
CHEMBL329066 1 6.70
CHEMBL13260 YM-90K 1 6.46
CHEMBL329496 1 6.44
CHEMBL98166 1 6.42
CHEMBL330594 1 6.41
CHEMBL318653 1 6.38
CHEMBL14935 TEZAMPANEL ANHYDROUS 2.0 1 6.22
CHEMBL421007 1 6.21
CHEMBL99228 1 6.21
CHEMBL279116 1 6.12
CHEMBL330632 1 6.08
CHEMBL319337 1 5.82
CHEMBL98879 1 5.80
CHEMBL319946 1 5.77
CHEMBL98816 1 5.64
CHEMBL316600 1 5.60
CHEMBL100155 1 5.47
CHEMBL328398 1 5.40
CHEMBL99663 1 5.37
CHEMBL98635 1 5.36
CHEMBL98820 1 5.15
CHEMBL317299 1 5.00
CHEMBL99587 1 5.00
CHEMBL318494 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL14712 IC50 = 86.0 nM 7.07
CHEMBL329066 IC50 = 200.0 nM 6.70
CHEMBL14712 IC50 = 200.0 nM 6.70
CHEMBL13260 YM-90K IC50 = 350.0 nM 6.46
CHEMBL329496 IC50 = 360.0 nM 6.44
CHEMBL98166 IC50 = 380.0 nM 6.42
CHEMBL330594 IC50 = 390.0 nM 6.41
CHEMBL318653 IC50 = 420.0 nM 6.38
CHEMBL14935 TEZAMPANEL ANHYDROUS IC50 = 600.0 nM 6.22
CHEMBL421007 IC50 = 610.0 nM 6.21
CHEMBL99228 IC50 = 620.0 nM 6.21
CHEMBL279116 IC50 = 760.0 nM 6.12
CHEMBL330632 IC50 = 830.0 nM 6.08
CHEMBL319337 IC50 = 1500.0 nM 5.82
CHEMBL98879 IC50 = 1600.0 nM 5.80
CHEMBL319946 IC50 = 1700.0 nM 5.77
CHEMBL98816 IC50 = 2300.0 nM 5.64
CHEMBL316600 IC50 = 2500.0 nM 5.60
CHEMBL100155 IC50 = 3400.0 nM 5.47
CHEMBL328398 IC50 = 4000.0 nM 5.40
CHEMBL99663 IC50 = 4300.0 nM 5.37
CHEMBL98635 IC50 = 4400.0 nM 5.36
CHEMBL14712 IC50 = 4900.0 nM 5.31
CHEMBL98820 IC50 = 7100.0 nM 5.15
CHEMBL317299 IC50 = 10000.0 nM 5.00
CHEMBL99587 IC50 = 10000.0 nM 5.00
CHEMBL318494 IC50 > 10000.0 nM -