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Assay Detail

CHEMBL700426

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of KinA/Sp0F system two component (TCS) from Bacillus subtilis.
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bacillus subtilis
Confidence 4 — Cell-line / Tissue
Curated By Expert

Target

KinA/Spo0F (sporulation kinase A/sporulation initiation phosphotransferase F) (CHEMBL2111331)
Type PROTEIN FAMILY
Organism Bacillus subtilis (strain 168)

Publication

Amidino benzimidazole inhibitors of bacterial two-component systems.
Weidner-Wells MA, Ohemeng KA, Nguyen VN, Fraga-Spano S, Macielag MJ, Werblood HM, Foleno BD, Webb GC, Barrett JF, Hlasta DJ.
Bioorg Med Chem Lett (2001) 11 : 1545 -1548
PubMed 11412977 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 4.63 5.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL288462 1 5.22
CHEMBL416972 1 5.11
CHEMBL38683 1 5.00
CHEMBL290098 1 5.00
CHEMBL39634 1 4.92
CHEMBL38914 1 4.85
CHEMBL38867 1 4.81
CHEMBL43691 1 4.77
CHEMBL39178 1 4.60
CHEMBL39040 1 4.36
CHEMBL290549 1 4.31
CHEMBL40096 1 4.19
CHEMBL417892 1 4.12
CHEMBL39543 1 4.07
CHEMBL2114423 1 4.06
CHEMBL289173 1 -
CHEMBL96917 1 -
CHEMBL433501 1 -
CHEMBL39218 1 -
CHEMBL39438 1 -
CHEMBL290462 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL288462 IC50 = 6000.0 nM 5.22
CHEMBL416972 IC50 = 7700.0 nM 5.11
CHEMBL38683 IC50 = 10000.0 nM 5.00
CHEMBL290098 IC50 = 10000.0 nM 5.00
CHEMBL39634 IC50 = 12000.0 nM 4.92
CHEMBL38914 IC50 = 14000.0 nM 4.85
CHEMBL38867 IC50 = 15400.0 nM 4.81
CHEMBL43691 IC50 = 17000.0 nM 4.77
CHEMBL39178 IC50 = 25000.0 nM 4.60
CHEMBL39040 IC50 = 44000.0 nM 4.36
CHEMBL290549 IC50 = 49000.0 nM 4.31
CHEMBL40096 IC50 = 64000.0 nM 4.19
CHEMBL417892 IC50 = 76000.0 nM 4.12
CHEMBL39543 IC50 = 86000.0 nM 4.07
CHEMBL2114423 IC50 = 88000.0 nM 4.06
CHEMBL289173 IC50 = 220000.0 nM -
CHEMBL96917 IC50 > 500000.0 nM -
CHEMBL433501 IC50 = 470000.0 nM -
CHEMBL39218 IC50 = 163000.0 nM -
CHEMBL39438 IC50 = 380000.0 nM -
CHEMBL290462 IC50 = 138000.0 nM -