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Assay Detail

CHEMBL701038

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Evaluated for the uptake of Methotrexate in L1210 leukemia cells
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210
Confidence 1 — Organism
Curated By Intermediate

Target

L1210 (CHEMBL386)
Type CELL-LINE
Organism Mus musculus

Publication

Chemistry and antitumor evaluation of selected classical 2,4-diaminoquinazoline analogues of folic acid.
Hynes JB, Harmon SJ, Floyd GG, Farrington M, Hart LD, Gale GR, Washtien WL, Susten SS, Freisheim JH.
J Med Chem (1985) 28 : 209 -215
PubMed 3968685 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.21 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 6.00
CHEMBL423123 1 5.80
CHEMBL305177 1 5.68
CHEMBL304397 1 5.54
CHEMBL305253 1 5.32
CHEMBL69713 1 4.13
CHEMBL303654 1 4.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 1000.0 nM 6.00
CHEMBL423123 IC50 = 1600.0 nM 5.80
CHEMBL305177 IC50 = 2100.0 nM 5.68
CHEMBL304397 IC50 = 2900.0 nM 5.54
CHEMBL305253 IC50 = 4800.0 nM 5.32
CHEMBL69713 IC50 = 74000.0 nM 4.13
CHEMBL303654 IC50 = 95000.0 nM 4.02