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Assay Detail

CHEMBL703613

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cell growth inhibition against mouse leukemia L1210/R71 cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210
Confidence 1 — Organism
Curated By Intermediate

Target

L1210 (CHEMBL386)
Type CELL-LINE
Organism Mus musculus

Publication

Methotrexate analogues. 25. Chemical and biological studies on the gamma-tert-butyl esters of methotrexate and aminopterin.
Rosowsky A, Freisheim JH, Bader H, Forsch RA, Susten SS, Cucchi CA, Frei E.
J Med Chem (1985) 28 : 660 -667
PubMed 3872941 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.92 5.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL346481 1 5.46
CHEMBL376180 AMINOPTERIN 2.0 1 5.10
CHEMBL34259 METHOTREXATE 4.0 1 4.72
CHEMBL422225 1 4.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL346481 IC50 = 3500.0 nM 5.46
CHEMBL376180 AMINOPTERIN IC50 = 7900.0 nM 5.10
CHEMBL34259 METHOTREXATE IC50 = 19000.0 nM 4.72
CHEMBL422225 IC50 = 40000.0 nM 4.40