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Assay Detail

CHEMBL703619

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Tested for cell-growth inhibition against mouse leukemic L1210/R81 cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210 ( R81)
Confidence 1 — Organism
Curated By Intermediate

Target

L1210 ( R81) (CHEMBL614710)
Type CELL-LINE
Organism Mus musculus

Publication

Methotrexate analogues. 34. Replacement of the glutamate moiety in methotrexate and aminopterin by long-chain 2-aminoalkanedioic acids.
Rosowsky A, Bader H, Kohler W, Freisheim JH, Moran RG.
J Med Chem (1988) 31 : 1338 -1344
PubMed 2898531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.17 4.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL26326 1 4.25
CHEMBL27306 1 4.24
CHEMBL283062 1 4.17
CHEMBL25923 1 4.14
CHEMBL28112 1 4.11
CHEMBL376180 AMINOPTERIN 2.0 1 4.08
CHEMBL26148 1 -
CHEMBL434004 1 -
CHEMBL34259 METHOTREXATE 4.0 1 -
CHEMBL275229 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL26326 IC50 = 56000.0 nM 4.25
CHEMBL27306 IC50 = 58000.0 nM 4.24
CHEMBL283062 IC50 = 68000.0 nM 4.17
CHEMBL25923 IC50 = 73000.0 nM 4.14
CHEMBL28112 IC50 = 78000.0 nM 4.11
CHEMBL376180 AMINOPTERIN IC50 = 84000.0 nM 4.08
CHEMBL26148 IC50 = 110000.0 nM -
CHEMBL434004 IC50 > 218000.0 nM -
CHEMBL34259 METHOTREXATE IC50 = 197000.0 nM -
CHEMBL275229 IC50 = 215000.0 nM -