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Assay Detail

CHEMBL705269

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Functional
Evaluated for cell growth inhibition of L1210 cells in cultures
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210
Confidence 1 — Organism
Curated By Intermediate

Target

L1210 (CHEMBL386)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis and antifolate activity of 5-methyl-5,10-dideaza analogues of aminopterin and folic acid and an alternative synthesis of 5,10-dideazatetrahydrofolic acid, a potent inhibitor of glycinamide ribonucleotide formyltransferase.
Piper JR, McCaleb GS, Montgomery JA, Kisliuk RL, Gaumont Y, Thorndike J, Sirotnak FM.
J Med Chem (1988) 31 : 2164 -2169
PubMed 3184124 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.86 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 8.68
CHEMBL69016 1 7.63
CHEMBL267890 1 7.24
CHEMBL66389 1 6.31
CHEMBL66864 1 4.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 2.1 nM 8.68
CHEMBL69016 IC50 = 23.2 nM 7.63
CHEMBL267890 IC50 = 57.2 nM 7.24
CHEMBL66389 IC50 = 490.0 nM 6.31
CHEMBL66864 IC50 = 36400.0 nM 4.44