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Assay Detail

CHEMBL706079

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Compound was tested for its ability to inhibit growth of L1210 mouse leukemia cells
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210
Confidence 1 — Organism
Curated By Intermediate

Target

L1210 (CHEMBL386)
Type CELL-LINE
Organism Mus musculus

Publication

Methotrexate analogues. 26. Inhibition of dihydrofolate reductase and folylpolyglutamate synthetase activity and in vitro tumor cell growth by methotrexate and aminopterin analogues containing a basic amino acid side chain.
Rosowsky A, Freisheim JH, Moran RG, Solan VC, Bader H, Wright JE, Radike-Smith M.
J Med Chem (1986) 29 : 655 -660
PubMed 2871191 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.79 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL376180 AMINOPTERIN 2.0 1 8.70
CHEMBL34259 METHOTREXATE 4.0 1 8.70
CHEMBL418165 1 6.40
CHEMBL158478 1 6.36
CHEMBL36083 1 5.89
CHEMBL280864 1 5.89
CHEMBL158970 1 5.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL376180 AMINOPTERIN IC50 = 2.0 nM 8.70
CHEMBL34259 METHOTREXATE IC50 = 2.0 nM 8.70
CHEMBL418165 IC50 = 400.0 nM 6.40
CHEMBL158478 IC50 = 440.0 nM 6.36
CHEMBL36083 IC50 = 1300.0 nM 5.89
CHEMBL280864 IC50 = 1300.0 nM 5.89
CHEMBL158970 IC50 = 2420.0 nM 5.62