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Assay Detail

CHEMBL707445

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
The compound was tested for inhibition of isolated thymidylate synthase partially purified from L1210 mouse leukemia cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type L1210
Confidence 1 — Organism
Curated By Intermediate

Target

L1210 (CHEMBL386)
Type CELL-LINE
Organism Mus musculus

Publication

Design and synthesis of potent non-polyglutamatable quinazoline antifolate thymidylate synthase inhibitors.
Marsham PR, Wardleworth JM, Boyle FT, Hennequin LF, Kimbell R, Brown M, Jackman AL.
J Med Chem (1999) 42 : 3809 -3820
PubMed 10508430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 8.18 9.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL126648 PLEVITREXED 2.0 1 9.36
CHEMBL128033 1 8.12
CHEMBL434209 1 8.00
CHEMBL389051 1 7.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL126648 PLEVITREXED Ki = 0.44 nM 9.36
CHEMBL128033 Ki = 7.5 nM 8.12
CHEMBL434209 Ki = 10.0 nM 8.00
CHEMBL389051 Ki = 60.0 nM 7.22