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Assay Detail

CHEMBL709249

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist concentration required to inhibit specific binding of [125I]leuprorelin to human luteinizing releasing hormone receptor in cloned chinese hamster ovary (CHO) cells.
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a thieno[2,3-d]pyrimidine-2,4-dione bearing a p-methoxyureidophenyl moiety at the 6-position: a highly potent and orally bioavailable non-peptide antagonist for the human luteinizing hormone-releasing hormone receptor.
Sasaki S, Cho N, Nara Y, Harada M, Endo S, Suzuki N, Furuya S, Fujino M.
J Med Chem (2003) 46 : 113 -124
PubMed 12502365 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 8.38 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL435167 1 10.00
CHEMBL22055 SUFUGOLIX 2.0 1 10.00
CHEMBL2092994 1 10.00
CHEMBL20511 1 9.70
CHEMBL21217 1 9.70
CHEMBL544440 1 9.70
CHEMBL280126 1 9.70
CHEMBL280365 1 9.52
CHEMBL21126 1 9.52
CHEMBL277214 1 9.40
CHEMBL280213 1 9.40
CHEMBL21936 1 9.22
CHEMBL280170 1 9.00
CHEMBL280212 1 9.00
CHEMBL21185 1 7.70
CHEMBL282679 1 7.70
CHEMBL279223 1 7.70
CHEMBL280350 1 7.52
CHEMBL21373 1 7.30
CHEMBL277435 1 7.10
CHEMBL281167 1 6.70
CHEMBL277653 1 6.70
CHEMBL277436 1 6.70
CHEMBL21804 1 6.40
CHEMBL21704 1 6.40
CHEMBL436346 1 6.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL435167 IC50 = 0.1 nM 10.00
CHEMBL22055 SUFUGOLIX IC50 = 0.1 nM 10.00
CHEMBL2092994 IC50 = 0.1 nM 10.00
CHEMBL20511 IC50 = 0.2 nM 9.70
CHEMBL21217 IC50 = 0.2 nM 9.70
CHEMBL544440 IC50 = 0.2 nM 9.70
CHEMBL280126 IC50 = 0.2 nM 9.70
CHEMBL280365 IC50 = 0.3 nM 9.52
CHEMBL21126 IC50 = 0.3 nM 9.52
CHEMBL277214 IC50 = 0.4 nM 9.40
CHEMBL280213 IC50 = 0.4 nM 9.40
CHEMBL21936 IC50 = 0.6 nM 9.22
CHEMBL280170 IC50 = 1.0 nM 9.00
CHEMBL280212 IC50 = 1.0 nM 9.00
CHEMBL21185 IC50 = 20.0 nM 7.70
CHEMBL282679 IC50 = 20.0 nM 7.70
CHEMBL279223 IC50 = 20.0 nM 7.70
CHEMBL280350 IC50 = 30.0 nM 7.52
CHEMBL21373 IC50 = 50.0 nM 7.30
CHEMBL277435 IC50 = 80.0 nM 7.10
CHEMBL281167 IC50 = 200.0 nM 6.70
CHEMBL277653 IC50 = 200.0 nM 6.70
CHEMBL277436 IC50 = 200.0 nM 6.70
CHEMBL21804 IC50 = 400.0 nM 6.40
CHEMBL21704 IC50 = 400.0 nM 6.40
CHEMBL436346 IC50 = 700.0 nM 6.16