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Assay Detail

CHEMBL711709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Tested in vitro against human recombinant Metabotropic glutamate receptor 5 stably expressed in LtK cells by [Ca2+] flux assay using glutamate as antagonist
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type LtK
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 5 (CHEMBL3227)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-[(2-Methyl-1,3-thiazol-4-yl)ethynyl]-pyridine: a potent and highly selective metabotropic glutamate subtype 5 receptor antagonist with anxiolytic activity.
Cosford ND, Tehrani L, Roppe J, Schweiger E, Smith ND, Anderson J, Bristow L, Brodkin J, Jiang X, McDonald I, Rao S, Washburn M, Varney MA.
J Med Chem (2003) 46 : 204 -206
PubMed 12519057 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.68 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL66654 1 8.70
CHEMBL292065 1 8.30
CHEMBL63042 1 8.22
CHEMBL65417 1 7.89
CHEMBL65022 1 7.28
CHEMBL65886 1 7.09
CHEMBL63093 1 7.01
CHEMBL303090 1 6.91

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL66654 IC50 = 2.0 nM 8.70
CHEMBL292065 IC50 = 5.0 nM 8.30
CHEMBL63042 IC50 = 6.0 nM 8.22
CHEMBL65417 IC50 = 13.0 nM 7.89
CHEMBL65022 IC50 = 53.0 nM 7.28
CHEMBL65886 IC50 = 81.0 nM 7.09
CHEMBL63093 IC50 = 97.0 nM 7.01
CHEMBL303090 IC50 = 122.0 nM 6.91