Assay Detail
Binding
CHEMBL745065
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Inhibition of binding of [3H]N-methylscopolamine to Muscarinic acetylcholine receptor M4 in NG 108-15 cell homogenates
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | NG 108-15 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL317) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Design, synthesis and biological activity of some tetraamines related to methoctramine and 4-DAMP
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 6 | 7.58 | 9.29 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL76897 | 4-DAMP | — | 1 | 9.29 |
| CHEMBL27673 | METHOCTRAMINE | — | 1 | 7.58 |
| CHEMBL165220 | — | — | 1 | 7.50 |
| CHEMBL9967 | PIRENZEPINE | 2.0 | 1 | 7.46 |
| CHEMBL163901 | — | — | 1 | 7.40 |
| CHEMBL194216 | SPIROTRAMINE | — | 1 | 6.27 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL76897 | 4-DAMP | Ki | = | 0.5129 | nM | 9.29 |
| CHEMBL27673 | METHOCTRAMINE | Ki | = | 26.3 | nM | 7.58 |
| CHEMBL165220 | — | Ki | = | 31.62 | nM | 7.50 |
| CHEMBL9967 | PIRENZEPINE | Ki | = | 34.67 | nM | 7.46 |
| CHEMBL163901 | — | Ki | = | 39.81 | nM | 7.40 |
| CHEMBL194216 | SPIROTRAMINE | Ki | = | 537.03 | nM | 6.27 |