Assay Detail
Functional
CHEMBL746612
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Inhibitory activity against [3H]-cyclic AMP accumulation in CHO transfected cells mediated by Muscarinic acetylcholine receptor M4
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Cell Type | CHO |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL317) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
6beta-Acetoxynortropane: a potent muscarinic agonist with apparent selectivity toward M2-receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.77 | 7.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL64000 | — | — | 1 | 7.96 |
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 6.64 |
| CHEMBL12587 | MUSCARINE | — | 1 | 6.33 |
| CHEMBL978 | METHACHOLINE | 4.0 | 1 | 6.16 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL64000 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL14 | CARBACHOL | IC50 | = | 230.0 | nM | 6.64 |
| CHEMBL12587 | MUSCARINE | IC50 | = | 470.0 | nM | 6.33 |
| CHEMBL978 | METHACHOLINE | IC50 | = | 700.0 | nM | 6.16 |