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Assay Detail

CHEMBL750484

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Nicotinic acetylcholine receptor binding activity was determined by ability to displace [3H]- (-) cytisine binding from whole rat brain synaptic membranes.
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Structure-activity studies on a novel series of cholinergic channel activators based on a heteroaryl ether framework.
Lin NH, Abreo MA, Gunn DE, Lebold SA, Lee EL, Wasicak JT, Hettinger AM, Daanen JF, Garvey DS, Campbell JE, Sullivan JP, Williams M, Arneric SP.
Bioorg Med Chem Lett (1999) 9 : 2747 -2752
PubMed 10509928 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 27 7.21 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL328705 1 9.89
CHEMBL84149 1 9.82
CHEMBL329682 1 8.62
CHEMBL329830 1 8.57
CHEMBL329004 1 8.35
CHEMBL93485 1 8.18
CHEMBL329138 1 7.93
CHEMBL92037 1 7.81
CHEMBL328575 1 7.79
CHEMBL94136 1 7.55
CHEMBL93026 1 7.54
CHEMBL92737 1 7.38
CHEMBL328367 1 7.33
CHEMBL92702 1 7.20
CHEMBL329768 1 7.07
CHEMBL92975 1 6.80
CHEMBL328429 1 6.62
CHEMBL90779 1 6.45
CHEMBL93459 1 6.39
CHEMBL96797 1 6.30
CHEMBL93922 1 6.25
CHEMBL93200 1 5.91
CHEMBL92085 1 5.89
CHEMBL94035 1 5.85
CHEMBL93146 1 5.10
CHEMBL329338 1 4.92
CHEMBL89943 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL328705 Ki = 0.13 nM 9.89
CHEMBL84149 Ki = 0.15 nM 9.82
CHEMBL329682 Ki = 2.39 nM 8.62
CHEMBL329830 Ki = 2.67 nM 8.57
CHEMBL329004 Ki = 4.44 nM 8.35
CHEMBL93485 Ki = 6.54 nM 8.18
CHEMBL329138 Ki = 11.7 nM 7.93
CHEMBL92037 Ki = 15.37 nM 7.81
CHEMBL328575 Ki = 16.3 nM 7.79
CHEMBL94136 Ki = 28.17 nM 7.55
CHEMBL93026 Ki = 29.0 nM 7.54
CHEMBL92737 Ki = 42.07 nM 7.38
CHEMBL328367 Ki = 46.4 nM 7.33
CHEMBL92702 Ki = 63.82 nM 7.20
CHEMBL329768 Ki = 85.35 nM 7.07
CHEMBL92975 Ki = 160.0 nM 6.80
CHEMBL328429 Ki = 240.0 nM 6.62
CHEMBL90779 Ki = 359.0 nM 6.45
CHEMBL93459 Ki = 406.0 nM 6.39
CHEMBL96797 Ki = 495.0 nM 6.30
CHEMBL93922 Ki = 560.0 nM 6.25
CHEMBL93200 Ki = 1223.0 nM 5.91
CHEMBL92085 Ki = 1300.0 nM 5.89
CHEMBL94035 Ki = 1400.0 nM 5.85
CHEMBL93146 Ki = 7914.0 nM 5.10
CHEMBL329338 Ki = 11900.0 nM 4.92
CHEMBL89943 Ki > 10000.0 nM -